Sunday 13 March 2016

2.11 describe experiments to investigate how enzyme activity can be affected by changes in temperature.

NOTE: Amylase is an enzyme which breaks down starch and iodine turns black when starch is present

- Put 30cm^3 of starch in six test tubes

- set up 5 water baths at different temperatures (e.g one at 10º, one at 15º, one at 20º, one at 25º and one at 30º) and put one test tube in each bath until they have all reached the desired temperature (keep one test tube in room temp for a control)
- add 10cm3 of amylase to each test tube (do not put amylase in the control test tube to ensure the starch does not break down without the amylase present)
- add iodine to each tube and time how long it takes for the starch to be digested (iodine will be blue/black with starch and return to normal colour, yellow/orange, once there is no starch present)
- To avoid anomalous results, repeat experiment three times.

Plot a graph of results

2 comments:

  1. Just to avoid confusion, I think you meant anomalous not anonymous. Thank you for putting up your notes by the way, they have been very helpful as I'm sitting my exam in this May. Good luck!

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  2. Yes I did, thank you for pointing it out, i'll change now it to avoid further confusion! It's okay & glad i helped, we're sitting them at the same time (: Good luck to you too! X

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