2.9 understand how the functioning of enzymes can be affected by changes in temperature, including changes due to change in active site

If temperatures are too high the enzyme will denature, meaning that it will not be able to function. This is because the energy breaks the bonds that holds the shape of the enzyme, therefore the active sight is no longer able to bind with the substrate.

However, there is an optimum temperature. This is the temperature that enzymes act best at, it is also just before the temperature at which the enzyme denatures.

The optimum temperature and temperature the enzyme denatures are different for every enzyme.